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Urease Antibody Peroxidase Conjugated
Catalog Number: orb344911
| Catalog Number | orb344911 |
|---|---|
| Category | Antibodies |
| Description | Urease antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, WB |
| Reactivity | Plant |
| Isotype | IgG |
| Immunogen | Urease [Jack Bean] |
| Concentration | 1 mg/mL |
| Dilution range | ELISA: 1:500 - 1:2,000, WB: 1:500 |
| Form/Appearance | Lyophilized |
| Purity | Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P07374 |
| NCBI | AAA83831.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Urease Antibody HRP Conjugation, Perox Read more... |
| Note | For research use only |
| Application notes | Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot Results of Rabbit Anti-Urease Antibody Peroxidase Conjugated. Urease (1) 33.33 ng, (2) 11.11 ng, (3) 3.70 ng, (4) 1.23 ng. Primary Antibody: Anti-Urease HRP at 1.0 µg/ml for 1hr at RT. Secondary Antibody: none. Blocking: BlockOut Buffer (orb348644) for 30 mins RT. Exposure: 5 secs.
