Urease antibody

• Catalog Number: orb344833
• Category: Antibodies
• Description: Urease antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Urease [Jack Bean]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P07374
• NCBI: AAA83831.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Urease Antibody, Urea Amidohydrolase a
• Note: For research use only
• Application notes: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Urease (Jack Bean) Antibody. Lane 1: Urease (Jack Bean). Load: 50 ng per lane. Primary antibody: Urease primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 90 kDa, 90 kDa for Urease.