Uplc1/Asap3 antibody

• Catalog Number: orb344637
• Category: Antibodies
• Description: Uplc1/Asap3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.
• Concentration: 1.10 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:10,000, WB: 1:200 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified antibody is directed against human UPLC1/ASAP3 protein. The product was purified from monospecific antiserum by protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with UPLC1/ASAP3 protein from mouse and rat based on approximately 80% homology with the human protein. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8TDY4
• NCBI: 19071867
• Storage: Store UPLC1 / ASAP3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-UPLC1 Antibody, rabbit anti-ASAP3 Anti
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to UPLC1/ASAP3 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein A purified anti-UPLC1/ASAP3 antibody shows detection of UPLC1/ASAP3 in NIH/3T3 cells over-expressing the protein. Cell extracts (5 ug) were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with anti-UPLC1/ASAP3 at a 1:10000 dilution.