Unconjugated Human PDGF-BB Protein

• Catalog Number: orb334919
• Category: Proteins
• Description: Unconjugated Human PDGF-BB, His, is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
• Reactivity: Human
• Tag: C-6×His & C-Avi
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 15.1 kDa
• Target: PDGF-BB
• UniProt ID: P01127
• Source: Unconjugated Human PDGF-BB, His, (orb334919) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Immobilized Unconjugated Human PDGF-BB, His, at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.5-16 ng/mL (QC tested).
• Expression Region: Ser 82 - Thr 190
• Endotoxins: 1.0 EU per μg
• NCBI: NP_002599.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 0.085% TFA in 30% ACN
• Alternative names: PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PDGF-B protein

Line graph illustrates about the sensitivity range of Human PDGF-B protein using different concentrations

Immobilized Unconjugated Human PDGF-BB, His, Avitag (Cat. No. orb334919) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. orb1146967) with a linear range of 0.5-16 ng/mL (QC tested).