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Catalog Number | orb1248877 |
---|---|
Category | Antibodies |
Description | UGT8 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine, Human, Rat |
Immunogen | The immunogen for this antibody is: C-NGKYKRNGHIK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml. Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | UGT8 |
UniProt ID | Q16880 |
NCBI | NP_003351.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydr Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml. Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Expiration Date | 12 months from date of receipt. |
MCF7 overexpressing Human UGT8 and probed with orb1248877 (mock transfection in first lane). Primary incubation (1 ug/ml) was overnight at 4C. Detected by chemiluminescence.
WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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