UCHL1 Antibody (C-term)

• Catalog Number: orb1931712
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21220
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Equine, Mouse, Porcine
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 24824 Da
• Target: This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
• UniProt ID: P09936
• NCBI: NP_004172.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ubiquitin carboxyl-terminal hydrolase isozyme L1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent image of U251 cell stained with UCHL1 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

UCHL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UCHL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UCHL1 Antibody (C-term) western blot analysis in U266, NCI-H1299, mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).