Ubiquitin-conjugating enzyme E2 B antibody

• Catalog Number: orb1151595
• Category: Antibodies
• Description: Mouse monoclonal antibody to Ubiquitin-conjugating enzyme E2 B
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-UBE2B-1C7
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human UBE2B protein was used as the immunogen for the UBE2B antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This UBE2B antibody is available for research use only.
• UniProt ID: P63146
• Storage: Aliquot the UBE2B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the UBE2B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using UBE2B antibody (green, clone PCRP-UBE2B-1C7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with UBE2B antibody (blue, clone PCRP-UBE2B-1C7), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using UBE2B antibody (clone PCRP-UBE2B-1C7). These results demonstrate the foremost specificity of the PCRP-UBE2B-1C7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.