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Catalog Number | orb1264994 |
---|---|
Category | Antibodies |
Description | UBE4A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Predicted Reactivity | Bovine, Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1037-1067 amino acids from the C-terminal region of human UBE4A. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 123 kDa |
Target | UBE4A |
UniProt ID | Q14139 |
NCBI | Q14139 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Ubiquitin conjugation factor E4 A, 632-, UBE4A (<a Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-UBE4A Antibody in NCI-H460 and CEM cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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