UBE2M antibody

• Catalog Number: orb578688
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE2M
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 21kDa
• Target: UBE2M
• UniProt ID: P61081
• Protein Sequence: Synthetic peptide located within the following region: IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISF
• NCBI: NP_003960
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: UBC12, hUbc12, UBC-RS2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Type: 2. mouse brain extracts (80 ug), Primary Antibody Dilution: 2 ug/ml, Secondary Antibody: IRDye 800CW goat anti-rabbit, Secondary Antibody Dilution: 1:20000.

Sample Type: NT2 cells, Red: Antibody, Blue: DAPI, Primary Dilution: 1 ug/50 ul antibody, Secondary Antibody: Alexa goat anti-rabbit 594.

Host: Rabbit, Target Name: UBE2M, Sample Type: Human 721_B, Antibody Dilution: 1.0 ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.

Host: Rabbit, Target Name: UBE2M, Sample Type: Human MCF7, Antibody Dilution: 1.0 ug/ml. UBE2M is supported by BioGPS gene expression data to be expressed in MCF7.

Sample Type: Human brain stem cells, Primary Antibody Dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody Dilution: 1:1000, Color/Signal Descriptions: UBE2M: Red DAPI:Blue, Gene Name: UBE2M.

WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate. UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.