UBE2L3 Antibody

• Catalog Number: orb1265293
• Category: Antibodies
• Description: UBE2L3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18 kDa
• Target: UBE2L3
• UniProt ID: P68036
• NCBI: P68036
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7,
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H.kidney section using UBE2L3 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis in K562, U87-MG, mouse NIH/3T3, rat PC-12 cell line and mouse testis, rat testis tissue lysates (35 ug/lane).