UBE2I Antibody

• Catalog Number: orb1672048
• Category: Antibodies
• Description: UBE2I Antibody
• Clonality: Recombinant
• Clone Number: SAIC-36A-9
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "GLFIIDGK", derived from the SUMO-conjugating enzyme UBC9, conjugated to KLH.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: UBE2I
• UniProt ID: P63279
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: SUMO-conjugating enzyme UBC9, UBC9, UBCE9
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-UBE2I antibody SAIC-36A-9. Paraformaldehyde fixed Jurkat cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-36A-9 (orb1672048 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurak cells with anti-UBE2I (SAIC-36A-9). Immunofluorescence analysis of paraformaldehyde fixed Jurak cells on Shi-fix™ cover-slips stained with the chimeric r version of SAIC-36A-9 (orb1672048) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672048- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.