UBE2I Antibody

• Catalog Number: orb1247876
• Category: Antibodies
• Description: UBE2I Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: SGIALSRLAQERK-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 17kDa band observed in Human Kidney lysates and in Rat Ovary, Heart and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration: 1ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UBE2I
• UniProt ID: P63279
• NCBI: NP_003336.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UBE2I, ubiquitin-conjugating enzyme E2I (UBC9 homo
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 17kDa band observed in Human Kidney lysates and in Rat Ovary, Heart and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration: 1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247876 (0.1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247876 (0.3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247876 (0.3 ug/ml) staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.