UBE2F Peptide

• Catalog Number: orb1236013
• Category: Proteins
• Description: UBE2F Peptide
• Tested applications: Blocking
• Form/Appearance: Lyophilized
• Target: UBE2F
• UniProt ID: Q969M7
• NCBI: NP_542409.1
• Storage: Store at -20°C. When reconstituted minimize freezing and thawing.
• Buffer/Preservatives: UBE2F peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
• Alternative names: NCE2, NCE2, NEDD8-conjugating enzyme UBE2F, NEDD8
• Note: For research use only
• Application notes: UBE2F Peptide is used for blocking the activity of UBE2F antibody (catalog number 42-421). The UBE2F antibody ships at 0.5 mg/mL and the UBE2F Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
• Expiration Date: 6 months from date of receipt.