UBE2D2 Antibody (N-term)

• Catalog Number: orb1788352
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: 44-76 aa
• Dilution range: WB: 1:2000, WB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 16735 Da
• Target: This UBE2D2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-76 amino acids from the N-terminal region of human UBE2D2.
• UniProt ID: P62837
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Hela, U-87 MG, MCF-7, SW480 cell line and rat testis tissue (from left to right), using UBE2D2 Antibody (N-term). Diluted at 1:500 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

All lanes: Anti-UBE2D2 Antibody (N-term) at 1:2000 dilution. Lane 1: F9 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: SW480 whole cell lysate. Lane 4: 293 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.