UBE2C Antibody

• Catalog Number: orb1672044
• Category: Antibodies
• Description: UBE2C Antibody
• Clonality: Recombinant
• Clone Number: SAIC-41A-1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: UBE2C
• UniProt ID: O00762
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ubiquitin-conjugating enzyme E2 C, UBCH10
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Caco-2 cells with anti-UBE2C (SAIC-41A-1). Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-41A-1 (orb1672044) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672044- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-UBE2C antibody SAIC-41A-1. Caco-2(A) (0.3 ug/ml)- MCF7(B) (1 ug/ml) and HeLa(C) (1 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-41A-1 (orb1672044) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the anti-UBE2C antibody SAIC-41A-1. Paraformaldehyde fixed MCF7 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-41A-1 (orb1672044 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.