UBC9 (UBE2I) Antibody (N-term)

• Catalog Number: orb1938824
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2811
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Other, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 18007 Da
• Target: This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).
• UniProt ID: P63279
• NCBI: NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: SUMO-conjugating enzyme UBC9, 632-, SUMO-protein l
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-UBE2I Antibody (N-term) in A2058 cell line lysates (35 ug/lane). UBE2I (arrow) was detected using the purified Pab.

Western blot analysis of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.