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Catalog Number | orb1520700 |
---|---|
Category | Antibodies |
Description | UAP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:5,000; IP - 3 - 6 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | UAP1 |
UniProt ID | Q16222 |
NCBI | Q16222.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | antigen X; UDP-N-acetylhexosamine pyrophosphorylas Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human UAP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UAP1 antibody (orb1520700) used for WB at 1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human UAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UAP1 antibody (orb1520700) used for IP at 6 µg per reaction.
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat, Yeast, Zebrafish | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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