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Catalog Number | orb1259784 |
---|---|
Category | Antibodies |
Description | TXN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TXN |
UniProt ID | P10599 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | TXN, ADF, ATL-derived factor, SASP, Thioredoxin, T Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TXN antibody (orb1259784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using TXN antibody (orb1259784) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using NFE2L1 antibody (orb1259805) at dilution of 1:100. Blue: DAPI for nuclear staining.
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
All | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, ICC, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |