Tubulin beta 3 antibody

• Catalog Number: orb542183
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Tubulin beta 3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human βIII-Tubulin/β3-Tubulin. (NP_006077.2).
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF: 1:50-1:200, IP: 1:50-1:200
• Conjugation: Unconjugated
• MW: 55kDa
• UniProt ID: Q13509
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraffin-embedded Mouse brain using [KO Validated] βIII-Tubulin Rabbit pAb (orb542183) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded Rat brain using [KO Validated] βIII-Tubulin Rabbit pAb (orb542183) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from wild type (WT) and βIII-Tubulin/β3-Tubulin knockout (KO) HeLa cells, using [KO Validated] βIII-Tubulin Rabbit pAb (orb542183) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of various lysates using [KO Validated] βIII-Tubulin Rabbit pAb (orb542183) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.