TUBA1C antibody

• Catalog Number: orb584734
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TUBA1C
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Predicted Reactivity: Canine, Porcine, Rat, Zebrafish
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBA1C
• UniProt ID: Q9NY65
• Protein Sequence: Synthetic peptide located within the following region: EAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEV
• NCBI: NP_116093
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: TUBA6, bcm948
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Type: 1. Human NT-2 cells (60 ug), 2. and 3. mouse brain extracts (80 ug), 4. rat brain extract (80 ug), Primary Antibody dilution: 2 ug/ml, Secondary Antibody: IRDye 800CW goat anti-rabbit, Secondary Antibody dilution: 1:20000.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.8 ug/ml of the antibody was used in this experiment. The protein may be modified by phosphorylation.

Amount and Sample Type: 500 ug mouse brain homogenate, Amount of IP Antibody: 6 ug, Primary Antibody: TUBA1C, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:5000, Gene Name: TUBA1C.

Sample Type: Human brain stem cells, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:1000, Color/Signal Descriptions: TUBA1C: Red DAPI:Blue, Gene Name: TUBA1C.

TUBA1C antibody - C-terminal region (orb584734) validated by WB using Placenta Lysate at 1.0 ug/ml.