TUBA1A Antibody

• Catalog Number: orb1672017
• Category: Antibodies
• Description: TUBA1A Antibody
• Clonality: Recombinant
• Clone Number: F2C
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: Human MBP (microtubule-binding protein).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TUBA1A
• UniProt ID: Q71U36
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TUBA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human cerebral cortex tissue using anti-Alpha Tubulin antibody. F2C Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of F2C (orb1672017). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1672017 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration- 2-4 ug/ml.

Western Blot using anti-Alpha-Tubulin antibody F2C. HeLa (A)- HEK293 (B)- and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of F2C (orb1672017) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-Tubulin is ~55kDa. orb1672017 successfully detected human Alpha-Tubulin in HeLa- HEK293- and HepG2 cell lysate samples.

Flow-cytometry using the anti-Alpha Tubulin F2C. HepG2 (A)- HeLa (B) and Hek293 (C) cells were stained with unimmunized r antibody (black line) or the rmeric version of F2C (orb1672017 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-Alpha-tubulin antibody F2C. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of F2C (orb1672017) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672017- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.