TTR Antibody / Transthyretin / Prealbumin

• Catalog Number: orb699785
• Category: Antibodies
• Description: Prealbumin, also designated transthyretin, is a major thyroid-hormone binding protein involved in transporting thyroxine from the bloodstream to the brain. Prealbumin is located in the cytoplasm and in the vesicles of developing rat brain cells, and is thought to be transported there from the cerebrospinal fluid via endocytosis. Sequence variants of this protein have been identified in amyloid fibrils from patients with familial amyloidotic polyneuropathy (FAP), the most common form of hereditary systemic amyloidosis. Although the biologically active form of Prealbumin is a tetramer, the amyloidogenic intermediate is thought to be a monomeric species. Prealbumin also binds to the retinol carrier protein RBP (retinol-binding protein).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TTR/4295
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Transthyretin antibody.
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Transthyretin antibody is available for research use only.
• UniProt ID: P02766
• Storage: Store the Transthyretin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Transthyretin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human liver lysate with Transthyretin antibody. Predicted molecular weight ~16 kDa.

IHC staining of FFPE human liver with Transthyretin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Transthyretin antibody. These results demonstrate the foremost specificity of the TTR/4295 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Transthyretin antibody as confirmation of integrity and purity.