TTF-1 / Thyroid Transcription Factor 1 / NKX2-1 antibody

• Catalog Number: orb2276213
• Category: Antibodies
• Description: TTF-1 / Thyroid Transcription Factor 1 / NKX2-1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-312R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human TTF-1 protein
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P43699
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BCH, Benign chorea, BHC, Homeobox protein Nkx2.1,
• Note: For research use only
• Application notes: Positive Control: Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control). Negative Control: Liver: TTF1 staining must not be seen in any cells. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Intense TTF1 positivity of pneumocytes.

Pulmonary adenocarcinoma with strong TTF1 staining of all tumor cells.

Thyroid gland with strong TTF1 staining of follicular epithelial cells.