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Catalog Number | orb2276212 |
---|---|
Category | Antibodies |
Description | TTF-1 / Thyroid Transcription Factor 1 / NKX2-1 antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | Biorbyt-312R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant human TTF-1 protein |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | P43699 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, Read more... |
Note | For research use only |
Application notes | Positive Control: Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control). Negative Control: Liver: TTF1 staining must not be seen in any cells. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Intense TTF1 positivity of pneumocytes.
Pulmonary adenocarcinoma with strong TTF1 staining of all tumor cells.
Thyroid gland with strong TTF1 staining of follicular epithelial cells.
IHC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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