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Catalog Number | orb1231206 |
---|---|
Category | Proteins |
Description | TSLP (R127A, R130A) Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Dilution range | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 16.7 kDa |
Target | TSLP |
UniProt ID | Q969D9 |
Protein Sequence | Tyr 29 - Gln 159 |
Source | HEK293 cells |
NCBI | R130, NP_149024.1, R127 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | TSLP Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human TSLP (R127A, R130A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human TSLP R, Fc Tag at 5 ug/ml (100 uL/well) can bind Human TSLP (R127A, R130A), His Tag with a linear range of 0.8-6 ng/mL.
Immobilized Human TSLP (R127A, R130A), His Tag at 1 ug/ml (100 uL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL.
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
41.6 kDa | |
HEK293 cells |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2136, Recombinant Human TSLP(R127A, R130A) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Tyr29-Gln159;(R127A, R130A)) of Human TSLP(R127A, R130A) fused with His Tag and Avi tag at the C-terminal. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2122, Biotinylated Recombinant Human TSLP(R127A, R130A) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Tyr29-Gln159;(R127A, R130A)) of Human TSLP(R127A, R130A) fused with His Tag and Avi tag at the C-terminal. |
Unconjugated | |
90% | |
16.6 kDa | |
Human TSLP Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1). |
Unconjugated | |
95% | |
41.6 kDa | |
Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag (orb612098) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)). |
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