Trypsin Inhibitor Antibody Peroxidase Conjugated

• Catalog Number: orb344907
• Category: Antibodies
• Description: Trypsin Inhibitor antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor (Soy Bean). Cross-reactivity against Trypsin Inhibitor from other tissues and species is unknown.
• Conjugation: HRP
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-trypsin inhibitor antibody HRP conjuga
• Note: For research use only
• Application notes: Anti-Trypsin Inhibitor peroxidase conjugated antibody has been tested by western blotting and ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti Trypsin Inhibitor antibody. Lane 1: purified Soybean Trypsin Inhibitor Reduced. Lane 2: purified Soybean Trypsin Inhibitor Non-Reduced. Lane 3: purified Streptavidin Reduced. Lane 4: purified Streptavidin Non-Reduced. Load: 1.0 ug per lane. Primary antibody: Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 25 kDa for Trypsin Inhibitor.

Western Blot of Rabbit Anti Trypsin Inhibitor antibody. Lane 1: purified Soybean Trypsin Inhibitor Reduced. Lane 2: purified Soybean Trypsin Inhibitor Non-Reduced. Lane 3: purified Streptavidin Reduced. Lane 4: purified Streptavidin Non-Reduced. Load: 1.0 ug per lane. Primary antibody: Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 25 kDa for Trypsin Inhibitor.