Trypsin Inhibitor antibody

• Catalog Number: orb344577
• Category: Antibodies
• Description: Trypsin Inhibitor antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Trypsin Inhibitor [Soybean]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin inhibitor antibody, 25 kDa try
• Note: For research use only
• Application notes: Soybean trypsin inhibitor antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Trypsin Inhibitor [Soybean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody. Lane 1: Opal PreStained Molecular Weight Marker. Lane 2: Trypsin Inhibitor [50 ng]. Primary Antibody: Anti-Trypsin Inhibitor at 1 µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Expect: ~25 kDa.