Trypsin Inhibitor antibody

• Catalog Number: orb344576
• Category: Antibodies
• Description: Trypsin Inhibitor antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Trypsin Inhibitor [Soybean]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin inhibitor antibody, 25 kDa try
• Note: For research use only
• Application notes: Soybean trypsin inhibitor antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Trypsin Inhibitor [Soybean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody. Lane 1: Opal PreStained Molecular Weight Marker. Lane 2: Trypsin Inhibitor [50 ng]. Primary Antibody: Anti-Trypsin Inhibitor at 1 µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Expect: ~25 kDa.