Trypsin Inhibitor Antibody Biotin Conjugated

• Catalog Number: orb344979
• Category: Antibodies
• Description: Trypsin Inhibitor antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin inhibitor antibody biotin conj
• Note: For research use only
• Application notes: Anti-Trypsin Inhibitor Biotin Conjugated antibody has been tested by ELISA and western blotting. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin. Other band(s): none.

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: Soybean Trypsin Inhibitor Reduced. Lane 2: Soybean Trypsin Inhibitor Non-Reduced. Lane 3: Streptavidin Reduced. Lane 4: Streptavidin Non-reduced. Load: ~1 ug per lane. Primary antibody: 1:1000 dilution of primary antibody (ON 4 C). Secondary antibody: Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT). Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa/20kDa for Trypsin, 18.8 kDa/15 kDa for Streptavidin. Other band(s): none.