Trypsin Inhibitor antibody (Biotin)

• Catalog Number: orb344987
• Category: Antibodies
• Description: Trypsin Inhibitor antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Trypsin Inhibitor [Lima Bean]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:500 - 1:2,000, WB: 1:250
• Form/Appearance: Lyophilized
• Purity: Anti-Trypsin Inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean]. Cross reactivity against Trypsin Inhibitor from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin inhibitor antibody biotin conj
• Note: For research use only
• Application notes: Anti-Trypsin Inhibitor antibody has been tested by dot blot and assayed against 1.0 ug of Trypsin Inhibitor in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:26,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Trypsin Inhibitor Antibody Biotin Conjugated. Dots are Trypsin Inhibitor at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit Anti-Trypsin Inhibitor Antibody Biotin at 1 µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.