Trypsin Inhibitor antibody (Biotin)

• Catalog Number: orb344980
• Category: Antibodies
• Description: Trypsin Inhibitor antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin inhibitor antibody biotin conj
• Note: For research use only
• Application notes: Anti-Trypsin Inhibitor Biotin Conjugated antibody has been tested by ELISA and western blotting. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin. Other band(s): none.

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: Soybean Trypsin Inhibitor Reduced. Lane 2: Soybean Trypsin Inhibitor Non-Reduced. Lane 3: Streptavidin Reduced. Lane 4: Streptavidin Non-reduced. Load: ~1 ug per lane. Primary antibody: 1:1000 dilution of primary antibody (ON 4 C). Secondary antibody: Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 24 kDa/20 kDa for Trypsin, 18.8 kDa/15 kDa for Streptavidin. Other band(s): none.