TRPM8 Antibody (C-term C940)

• Catalog Number: orb1928520
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14420
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:500, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 127685 Da
• Target: This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 926-956 amino acids from the C-terminal region of human TRPM8.
• UniProt ID: Q7Z2W7
• NCBI: NP_076985.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transient receptor potential cation channel subfam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (C-term C940).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2).

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:500 dilution. Lane 1: LNCap whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 125kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:1000 dilution. Lane 1: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 128kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:1000 dilution. Lane 1: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 125kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (C-term C940), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.