TRPA1 antibody

• Catalog Number: orb575403
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TRPA1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Yeast
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRPA1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 127 kDa
• Target: TRPA1
• UniProt ID: O75762
• Protein Sequence: Synthetic peptide located within the following region: KCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLAL
• NCBI: NP_015628
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: FEPS, FEPS1, ANKTM1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1.5 ug/ml of the antibody was used in this experiment. Protein may be modified by glycosylation.

Host: Rabbit, Target Name: TRPA1, Sample Tissue: Human HepG2, Antibody dilution: 1.0 ug/ml.

Host: Rabbit, Target Name: TRPA1, Sample Tissue: Human THP-1 Whole Cell, Antibody dilution: 1 ug/ml.

Immunohistochemistry with rat sensory ganglia tissue.

Sample Type: Section of TRPM8-GFP transgenic mouse trigeminal ganglia, Primary Antibody dilution: 1:250, Secondary Antibody: Goat Anti-Rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:1000, Color/Signal Descriptions: TRPA1: Red TRPM8-GFP:: Green, Gene Name: TRPA1.

WB Suggested Anti-TRPA1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Human Stomach.