TRP1 / Tyrosinase-Related Protein-1 Antibody

• Catalog Number: orb606412
• Category: Antibodies
• Description: Tyrosinase-Related Protein-1 / TYRP1 / TRP1 is involved in melanin synthesis. It is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. It is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TYRP1/2340R
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 257-377 was used as the immunogen for this recombinant TRP1 antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant TRP1 antibody is available for research use only.
• UniProt ID: P17643
• Storage: Store the recombinant TRP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant TRP1 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human basal cell carcinoma with recombinant TRP1 antibody (clone TYPR1/2340R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human melanoma with recombinant TRP1 antibody (clone TYPR1/2340R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human melanoma with recombinant TRP1 antibody (clone TYPR1/2340R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant TRP1 antibody (clone TYRP1/2340R) as confirmation of integrity and purity.