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Catalog Number | orb767264 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Troponin I-C (phospho-Ser22/S23) |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 28 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-TNNI3 antibody, anti-TNNC1 antibody, anti-Tro Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of SH-SY5Y cells using Phospho-Troponin I-C (S22/S23) Polyclonal Antibody diluted at 1:1000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TNNI3 (Phospho-Ser22 + Ser23) Antibody.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using TNNI3 (Phospho-Ser22 + Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from mouse heart, using TNNI3 (Phospho-Ser22 + Ser23) Antibody. The lane on the right is blocked with the phospho peptide.