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Catalog Number | orb1806457 |
---|---|
Category | Antibodies |
Description | Troponin I Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1 and 50 |
Concentration | 250 µg/ml |
Dilution range | WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Troponin I |
UniProt ID | P19429 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | RCM1; troponin I, cardiac muscle; troponin I type Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse Troponin I by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, heart, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.
Detection of human Troponin I by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, heart, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.
Detection of human Troponin I by immunohistochemistry. Sample: FFPE section of human cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse Troponin I by immunohistochemistry. Sample: FFPE section of mouse cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457). Secondary: HRP-conjugated goat anti-rabbit IgG.
ELISA, FC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC-Fr, IHC-P, WB | |
Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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