Trk B (phospho Tyr706) rabbit pAb

• Catalog Number: orb769305
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Trk B (phospho-Tyr706)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr705. AA range:671-720
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 92
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NTRK2 antibody, anti-TRKB antibody, anti-BDNF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Trk B (phospho Tyr706) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of 293 cells using Phospho-Trk B (Y706) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B (Phospho-Tyr705) Antibody.

Immunofluorescence analysis of HUVEC cells, using Trk B (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.