Trk B (phospho Tyr516) rabbit pAb

• Catalog Number: orb769304
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Trk B (phospho-Tyr516)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr515. AA range:481-530
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 145
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NTRK2 antibody, anti-TRKB antibody, anti-BDNF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of RAT-MUSCLE cells using Phospho-Trk B (Y516) Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B (Phospho-Tyr515) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.