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Catalog Number | orb137146 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to TriMethyl-Histone H3-K27 |
Clonality | Polyclonal |
Tested applications | ChIP, ICC, IF, IHC, IP, WB |
Reactivity | All, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic trimethylated peptide around K27 of human histone H3(NP_003520.1). |
Concentration | 1 mg/ml |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100, ChIP |
Conjugation | Unconjugated |
MW | 17KDa |
UniProt ID | Q16695 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Alternative names | anti HIST1H3J antibody, anti H3/j antibody, anti H Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Cheng, Saifeng et al. WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice Nucleic Acids Res, 46, 2356-2369 (2018)
Immunohistochemical staining of paraffin embedded rat testis tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)
IHC-P image of rat brain tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)
IHC-P staining of human thyroid cancer tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human rectal cancer tissue using Histone Histone H3K27me3 antibody (primary antibody dilution at 1:200)
Immunofluorescense analysis of 293T cell using Histone Histone H3K27me3 antibody
Dot-blot analysis of all sorts of methylation peptides using H3K27me3 antibody
Chromatin Immunoprecipitation analysis of actin gene from 293 cell line TriMethyl-Histone H3-K27 antibody
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli using Histone Histone H3K27me3 antibody
Immunoprecipitation analysis of various genes from 293 cell line using Histone Histone H3K27me3 antibody
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