TRIM5 Antibody

• Catalog Number: orb1238676
• Category: Antibodies
• Description: TRIM5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta.The immunogen is located within the last 50 amino acids of TRIM5 delta.
• Concentration: 1 mg/mL
• Dilution range: TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 36 kDa Observed: 33 kDa
• Target: TRIM5
• UniProt ID: Q9C035
• NCBI: NP_149084
• Storage: TRIM5 delta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TRIM5 delta Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TRIM5 delta Antibody: RNF88, TRIM5alpha, RNF88, Tr
• Note: For research use only
• Application notes: TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRIM5 delta in human kidney tissue lysate with TRIM5 delta antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 1 µg/mL.

Immunofluorescence of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 10 µg/mL.