TRIM25 antibody

• Catalog Number: orb1474482
• Category: Antibodies
• Description: Rabbit monoclonal antibody to TRIM25
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Rat
• Immunogen: A synthetic peptide of human TRIM25
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: TRIM25
• Entrez: 7706
• UniProt ID: Q14258
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRIM25 expression in K562 (A), rat brain (B), C6 (C), Hela (D) whole cell lysates. (Predicted band size: 71 kD; Observed band size: 71 kD)

Immunofluorescent analysis of TRIM25 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.