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Catalog Number | orb345193 |
---|---|
Category | Antibodies |
Description | Transferrin antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Transferrin Antibody was produced by repeated immunizations with human serum Transferrin protein. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | Anti-Transferrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum]. Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined. |
Conjugation | Unconjugated |
UniProt ID | P02787 |
NCBI | AAH59367.1 |
Storage | Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Transferrin Antibody, Apotransferrin a Read more... |
Note | For research use only |
Application notes | Anti-Transferrin Antibody has been tested in western blotting, ELISA and IHC. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Rabbit anti human Transferrin antibody. Tissue: Proliferant, cycling human thymus. Antigen Retrieval: HIER pH6.2. Staining: diffuse cytoplasmic and membrane staining. Right: Transferrin antibody at 1:100. Left: Normal Rabbit IgG Neg Ctrl.
Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue)secondary antibody at 1:10000 for 45 min at RT. Block: IRdye blocking buffer (orb348637) 1hr at 4°C. Other band(s): none.
Western Blot of Rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Other band(s): none.
Western Blot of Rabbit Anti-Transferrin primary antibody. Lane 1: Human Transferrin. Lane 2: None. Primary antibody: Transferrin primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 76-81 kDa. 76 kDa for Transferrin. Other band(s): Transferrin slice varients and isoforms.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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