Transcription factor E3 / TFE3 antibody

• Catalog Number: orb2276367
• Category: Antibodies
• Description: Transcription factor E3 / TFE3 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-403R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human TFE3 fragment
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P19532
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BHLHE33
• Note: For research use only
• Application notes: Positive Control: Kidney: A moderate to strong TFE3 staining should be seen in a fraction of glomerular cells and an at least weak TFE3 staining should be seen in a subset of tubular and collecting duct cells. Negative Control: Colon: TFE3 staining should be absent in epithelial cells (some very faint staining can occasionally occur at the surface). Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Strong nuclear TFE3 positivity in a renal cell carcinoma with TFE3 fusion.

Strong TFE3 staining of epithelial cells in the corpus epididymis.

TFE3 staining is lacking in epithelial cells of the appendix.