TP53 Antibody

• Catalog Number: orb1274258
• Category: Antibodies
• Description: TP53 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TP53/1799R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG, kappa
• Immunogen: Recombinant human p53 protein was used as the immunogen for this recombinant p53 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TP53
• UniProt ID: P04637
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Cellular tumor antigen p53, Antigen NY-CO-13, Phos
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using recombinant p53 antibody (clone TP53/1799R). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human colon carcinoma with recombinant p53 antibody (clone TP53/1799R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone TP53/1799R).