TP53 Antibody

• Catalog Number: orb1273516
• Category: Antibodies
• Description: TP53 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Monkey
• Immunogen: p53 (Ser392) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53.
• Concentration: batch dependent
• Dilution range: Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53
• Target: TP53
• UniProt ID: P04637
• NCBI: P04637
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of rat brain fraction lysate showing phosphospecific immunolabeling of the ~53k p53 protein phosphorylated at Ser392.