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Catalog Number | orb1474366 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to TORC1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, IF, IP, WB |
Reactivity | Human |
Immunogen | Purified recombinant human MECT1 / Torc1 protein fragments expressed in E.coli. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100) |
Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Conjugation | Unconjugated |
Target | CRTC1 |
Entrez | 23373 |
UniProt ID | Q6UUV9 |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Alternative names | KIAA0616; MECT1; TORC1; WAMTP1; CREB-regulated tra Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of TORC1 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 78 kD)
Immunofluorescent analysis of TORC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Flow cytometric analysis of TORC1 in K562 cells (red). Blue line histogram represents the isotype control.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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