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Catalog Number | orb1331907 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Topoisomerase II alpha (TOP2A) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | OTI1D2 |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Human recombinant protein fragment corresponding to amino acids 1100-1531 of human TOP2A(NP_001058) produced in E.coli. |
Dilution range | WB 1:2000, IHC 1:150 |
Conjugation | Unconjugated |
MW | 174.2 kDa |
Target | TOP2A |
UniProt ID | P11388 |
NCBI | NM_001067 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (Right lane) cDNA for 48 hrs and lysed using Topoisomerase II alpha (TOP2A) antibody
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Hamster, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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