Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb750178
• Category: Antibodies
• Description: This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TPM2A-1
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TOP2A antibody is available for research use only.
• Entrez: 7153
• UniProt ID: P11388
• Storage: Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with TOP2A antibody (clone TPM2A-1).

IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TPM2A-1).

Immunofluorescent staining of PFA-fixed human HeLa cells with TOP2A antibody (clone TPM2A-1).

Western blot testing of human HeLa cell lysate with TOP2A antibody (clone TPM2A-1). Expected molecular weight ~174 kDa.