Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb750075
• Category: Antibodies
• Description: This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TOP2A/1362
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TOP2A antibody is available for research use only.
• Entrez: 7153
• UniProt ID: P11388
• Storage: Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human HeLa cells with TOP2A antibody (green, clone TOP2A/1362) and Phalloidin (red).

Western blot testing of human HeLa cell lysate with TOP2A antibody (clone TOP2A/1362). Expected molecular weight ~174 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TOP2A antibody (clone TOP2A/1362). These results demonstrate the foremost specificity of the TOP2A/1362 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TOP2A antibody (clone TOP2A/1362) as confirmation of integrity and purity.