Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb699556
• Category: Antibodies
• Description: The topoisomerase II is the predominant mechanism of resistance to several chemotherapeutic agents.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TOP2A/4397R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 1431-1531 from the human protein was used as the immunogen for the recombinant TOP2A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant TOP2A antibody is available for research use only.
• UniProt ID: P11388
• Storage: Store the recombinant TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with recombinant TOP2A antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node with recombinant TOP2A antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.